Protein_Domain

Part:BBa_K1733000:Design

Designed by: Jeffrey Chen   Group: iGEM15_BostonU   (2015-09-17)


orf7 Recombination Directionality Factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 186


Design Notes

Since this protein is extremely small, it was already biobrick compatible and we were also able to test it in mammalian systems. We simply put this protein into the psb1c3 backbone through a PCR reaction, in order to add the biobrick suffix and prefix, and ligation.

Source

Porcine Reproductive and Respiratory Syndrome Virus

References